Arrayed CRISPR Libraries
transEDIT Arrayed CRISPR Libraries
- Enabling single-target or combinatorial vector-based genetic screens at scale in arrayed format
- sgRNAs designed by CRoatan algorithm – read the publication in Molecular Cell
- Highly efficient lentiviral vector backbone for producing high-titer lentiviral particles
- Exceptionally bright ZsGreen fluorescent reporter
- All CRISPR library sgRNA constructs are 100% sequence-confirmed
- Order premade or custom arrayed CRISPR Libraries
transEDIT CRISPR Arrayed Libraries allow single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. sgRNAs are designed using the CRoatan algorithm which is based on a machine-learning approach combined with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system.
The only commercially available Dual sgRNA Arrayed CRISPR KO Library:
The transEDIT Dual sgRNA Arrayed CRISPR KO Library covering the Human Genome was developed by transOMIC in partnership with Cold Spring Harbor Laboratory using the CRoatan algorithm and a multiplexed sgRNA expression for combinatorial targeting. These strategies have been combined to design and construct a human genome-wide, sequence-verified, arrayed CRISPR library, employing the enhancement effect of co-expressing two different sgRNAs for the same target:
You can purchase the whole genome wide arrayed dual sgRNA library (Click here to download the gene list) or you can pick and choose for building a custom arrayed library.
transEDIT Custom Arrayed CRISPR Libraries: Any size – Any application
Our partner transOMIC Technologies offers maximal flexibility for your arrayed CRISPR screening project, based on their wealth of vectorology, and clone handling experience.
transOMIC pCLIP CRISPR Vectors:
- Build your custom CRISPR array cloned in any of transOMIC´s pCLIP lentiviral/retroviral vectors for CRISPR KO, CRISPRa, or CRISPRi. If you prefer your own lentiviral vector, we can accomodate this, too!
- sgRNAs for your target gene list will be designed using the CRoatan algorithm or you can share your own sgRNA designs
- Oligos are synthesized and cloned into your choice of lentiviral vector.
- Each clone is sequence-verified and arrayed into 96-well plates.
- We can deliver glycerol stocks, plasmid DNA, and/or ready-to-use lentiviral particles in 96-well plates.
Please contact us with your project details and share your gene list, the number of sgRNAs needed for targeting each gene, and your vector choice with us. We will be pleased to send you a detailed quotation.
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