Premade Lentiviral ORF Expression Constructs

MGC premier LentiORF Clone Collection: Premade lentiviral ORF expression constructs for 11,373 human genes

In cooperation with our partner transOMIC we are offering the complete MGC premier LentiORF arrayed library as well as individual constructs chosen by you.

• Pre-cloned and sequence-verified lentiviral ORF clones significantly speed up your research
• Choose from different formats: Glycerol Stock, Transfection Grade Plasmid DNA, Ready-To-Transduce Lentiviral Particles
• MGC Premier ORF clones have been the gold standard used by the scientific community for over 20 years as shown in numerous publications
• pLX304 Lentiviral ORF Expression vector: V5 tagged ORF expression driven by CMV promoter; ORF flanking Gateway sites
• Customized subcloning in other expression vector of your choice

This Human Lentiviral ORF Expression Library was developed through the collaborative efforts of Dana-Farber Cancer Institute, The Broad Institute and the Center for Cancer Systems Biology (CCSB). The collection, which includes 13,579 ORF clones representing 11,373 genes, provides the most fully sequenced and annotated version of the human ORFeome available. Click here to download the data file featuring all constructs.

Schematic depicting the elements of the lentiviral vector pLX304 expressing the V5 tagged ORF

For added convenience the lentiviral ORF expression vector pLX304 (For detailed map and sequence click here) was created to enable expression of a protein of interest with a V5 fusion tag for western blot detection, puri­fication, co-immunoprecipitation, protein localization and FACS analysis.

Custom Lentiviral ORF Expression Cloning Service:

You like the ORF to be expressed in a different lentiviral vector? Sure!

transOMIC offers both constitutive and inducible lentiviral ORF expression vectors in a modular manner with different promoters for driving expression as well as different fluorescence and resistance markers:

transOMIC Lentiviral Vector Options for Constitutive ORF Expression

transOMIC Lentiviral Vector Options for Inducible ORF Expression

Just let us know which ORF(s) you like to be subcloned, how your vector should look like, and if you like glycerol stock, plasmid DNA, or particles to be delivered and we will send you a quote.

You like the ORF to be modified – e.g. codon-optimized or mutated, or you don´t find the gene of your interest in the data file of the MGC Premier Lentiviral Human ORF collection?

No problem! transOMIC also offers a gene synthesis and subsequent cloning service. Please contact us and tell us your requirements to receive a quote!