RNA Methylation Sequencing: Enhanced m6A-seq/meRIP-seq
High Resolution Maps of the Epitranscriptome – Efficient and precise identification of m6A modification sites
N6-Methyladenosine (m6A) is the most abundant modified nucleoside in mRNA. The dynamic epitranscriptomic m6A RNA modification by writers and erasers and its recognition by readers is regulating cellular and physiological processes and is involved in many diseases.
Eclipse BioInnovation´s m6A-eCLIP-seq Kit is an NGS-based assay for the most sensitive, accurate and reproducible detection of m6A modification sites, superior to meRIP-seq protocols:
⇒ Accurate Single Nucleotide Resolution
Reverse transcriptase (RT) termination at the crosslink sites of the m6A antibody to RNA form sharp cliffs of aligned read starts enabling single-nucleotide resolution modification calls. More than 70% of identified sites are positioned on adenosine nucleotides, and over 50% of all crosslink sites map precisely to adenosines in the DRACH motif preferred by m6A methyltransferases
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