transEDIT Lentiviral Dual sgRNA Arrayed CRISPR KO Whole Genome Library, Human – 60987 constructs targeting >18000 genes , glycerol stocks

Description

transEDIT Dual sgRNA Arrayed CRISPR KO Library: 60987 premade arrayed dual sgRNA constructs for > 18,000 human genes

Price shown is for academic institutions; commercial entities please inquire for a quotation

Contents: 670 96-well plates (250ul in each well)

In cooperation with our partner transOMIC we are offering the complete transEDIT arrayed dual sgRNA CRISPR KO library targeting the human genome:

• Enabling single-target or combinatorial genetic screens at scale in arrayed format
• 3-4 individually arrayed dual sgRNA constructs for each target gene
• sgRNAs designed by CRoatan algorithm – read the publication in Molecular Cell
• Over 1,000 negative controls are contained in the arrayed library
• Highly efficient  pCLIP-dual-SFFV-ZsGreen-Puromycin vector backbone for producing high-titer lentiviral particles
• Each dual sgRNA construct contains a unique barcode, a puromycin selection marker and the exceptionally bright ZsGreen fluorescent reporter
• All CRISPR KO library dual sgRNA constructs are pre-cloned and 100% sequence-confirmed
• Delivered as individual glycerol stocks, arrayed in 96-well plates

The only commercially available Dual sgRNA Arrayed CRISPR KO Library:

The transEDIT Dual sgRNA Arrayed CRISPR KO Library covering the Human Genome was developed by transOMIC in partnership with Cold Spring Harbor Laboratory using the CRoatan algorithm and a multiplexed sgRNA expression for combinatorial targeting. These strategies have been combined to design and construct a human genome-wide, sequence-verified, arrayed CRISPR library, employing the enhancement effect of co-expressing two different sgRNAs for the same target.

You can purchase the whole genome wide arrayed dual sgRNA library (Click here to download the gene list) or you can pick and choose for building a custom arrayed library, or order individual dual sgRNA constructs. Please contact us with your wish list.