
Get results from gene screens with pooled CRISPR, shRNA, and barcode libraries faster by letting Cellecta do the NGS prep, sequencing, and analysis.
If you are running your own pooled lentiviral gene screens with CRISPR sgRNA, RNAi hairpin, or cell barcode labeling library just provide harvested cells or genomic DNA for each time point or treatment condition (one sample), and Cellecta will provide you with a spreadsheet enumerating the read number for each of the library elements. The Genetic Screen Sequencing Services includes:
- Extraction of genomic DNA (optional)
- Amplification of barcodes or sgRNA
- NGS on the Illumina NextSeq or HiSeq
- Enumeration of shRNA, sgRNA, or barcode counts from raw sequencing data
- Additional bioinformatics and pathway analysis available on request.
This service is provided not only for Cellecta libraries but also similar non-Cellecta CRISPR libraries, such as
Take advantage of Cellectas extensive experience in designing primer and running NGS on a large variety of pooled libraries for various types of genetic screens. Simply ship us frozen cell pellets, tissue or genomic DNA from each time point or treatment condition and Cellecta does the rest!
Nucleus Biotech will be your partner in organizing the sequencing service of samples from your genetic screen.
Please use our Service request form to contact us for more details or request a quote