Choose your number of defined barcodes.
Cellectas off-the-shelf libraries with 1M, 10M, and 50M barcodes may be too complex for your experimental needs. Some studies looking at population distributions may only require a few thousand barcodes. We can certainly provide smaller libraries with 1000, 5000 or 20,000 unique, distinct barcodes. With these libraries of limited complexity, deeper sequencing of the whole library is possible.
Specialized markers or selection
For some barcode tracking experiments, a different marker or selection may be required, such as Thy1.1, luciferase, or thymidine kinase. We can readily generate a barcode library of any size in a specialized vector for you.
Barcoding combined with other effectors or elements in a library. As deep sequencing has become much more accessible, barcode tracking has become a useful tool for tracking cell-to-cell variations in conjunction with a variety of assays. For example, several groups have focused on using barcode tracking to assay RNA expression in conjunction with pooled CRISPR screening (aka., Perturb-Seq, CRISPR-Seq, CROP-Seq). Cellecta is able to provide libraries that combine barcodes with sgRNA, shRNA, etc. and offers the pScribe Vectors so that the barcodes are transcribed and trackable by RNA sequencing, as well as genomic DNA analysis.
Cell-specific barcodes incorporated into pooled CRISPR sgRNA libraries can be used in conjunction with single-cell RNA expression analysis to perform Perturb-Seq / CRISPR-Seq screens. The approach enables researchers to reliably identify and link changes in gene expression to the knockout of particular target genes.
Nucleus Biotech will be more than happy to assist you to find the custom lentiviral barcode library that enable you to generate populations of millions of cells where each have their own uniquely identifiable barcode sequence.