Cas9 Expression Constructs

For the standard CRISPR system, knockout efficiency is directly dependent on Cas9 expression levels and transient expression is not reliable for genetic screens with pooled sgRNA libraries. For CRISPR screening purposes, it is important to use cells that express Cas9 well. Further, Cas9 expression levels definitely vary from cell type to cell type, depending on the promoter used to drive Cas9 expession and the number of Cas9 copies per cell. To ensure optimal expression in your cell model for screening and gene editing, Nucleus Biotech offers a range of different Cas9 and dCas9-variant expressing constructs.

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