Cas9 Proteins

The use of CasRNPs is an improvement on plasmid transfections because the Cas protein is cleared from the system within 24 hours, decreasing off-target effects. The method also saves time, as the active complex is what’s being delivered straight to the nucleus of the cell. Purity of Cas9 highly impacts the efficiency of CRISPR-based genome editing using the RNP approach. We are offering the highly purified wildtype S. pyogenes Cas9 protein with two (N-terminal and C-terminal) nuclear localization signals (NLS) from our partner TriAltus Bioscience who used their ultra-high-affinity CL7/lm7 chromatography system to yield highly pure and active Cas9 in contrast to the traditionally used his tag purification:

Researchers at Hubei University (Wuhan, China) used Trialtus´ CL7/Im7 system to purify even Cas9 ribonucleoproteins (RNPs) in one step (Qiao et al.: Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins. Commun Biol 2, 161 (2019).

We are also pleased to offer purified recombinant S. pyogenes Cas9 protein in various package sizes from our supplier Macrogen, which can be used along with Marcogen´s custom synthesized sgRNA to form transfectable/injectable Cas9 RNP complexes.

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