Custom sgRNA Constructs

Lentiviral vectors are one of the most convenient and effective vehicles to introduce and stably express your sgRNA in almost any mammalian cell.
Our partner Cellecta offers improved HEAT designed custom sgRNAs suitable for gene knockout, promoter activation, promoter repression or  knock-ins.

All  guides are  cross-checked against the UCSC CRISPR targets track to ensure they are unique in the genome. The UCSC specificity quartile is used to score for off-target potential, and the Doench 2016 Azimuth activity scores to rank the guides for efficacy. This special sgRNA design was proven to generate more robust screening results than the standard sgRNA designs.
In order to accelerate your research, take advantage of the expertise of our partner Cellecta:

  • Flexible design services to ensure the sgRNA target your genes, isoforms, or regulatory elements
  • Range of vector designs, including sgRNA-only, sgRNA-Cas9, dual-sgRNA and tet-inducible sgRNA vectors
  • Get lentiviral plasmids and pre-packaged lentiviral particles
  • Control guides available in any vector backbone

Just provide information about your gene of interest and Cellecta will design, clone and deliver the optimal CRISPR sgRNA lentiviral constructs according to your needs.
Nucleus Biotech works with you to optimize all aspects of the sgRNA constructs you need.
Please use our Service request form  to contact us for more details or request a quote.

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