Pooled CRISPR KO Libraries

Pooled lentiviral CRISPR sgRNA libraries contain many thousands of defined single-guide RNA sequences enable disruption (“knockout”) of target genes throughout a mammalian cell population in a single experiment. Screening this cell population for a specific phenotype (e.g., viability) then, enables the identification of specific genes driving this phenotype of interest.
Nucleus Biotech provides human and mouse whole genome pooled KO libraries as well as separate modules covering different pathways and approved drug targets. All libraries are offered as plasmid and pre-packaged lentivirus.

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