Pooled CRISPRi Libraries

CRISPR interference (CRISPRi) targeted gene repression systems provide alternative genetic screening approaches to identify genes required to maintain a biological response (loss-of-function screen). CRISPRi libraries that repress endogenous gene expression, make use of engineered versions of deactivated Cas9 (dCas9) which have proven to be effective modulators of gene expression when complexed with repressor (e.g., dCas9-KRAB) proteins.
Nucleus Biotech provides Cellecta´s pre-made, pooled lentiviral libraries targeting the promoter regions of all human or mouse protein-coding genes.

Unique are Cellecta´s Dual sgRNA CRISPRi libraries:  Targeting each gene with two sgRNAs for its promoter region means stronger repression.


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