Cas12b Proteins
Recombinant Cas12b Protein for High-Fidelity CRISPR Applications
• Recombinant AapCas12 for forming RNP complexes with guide-RNA
• Highly purified and extremely active
• Trans ssDNA cleavage activity leads to highly increased specificity
Cas12b Nucleases belong to type V CRISPR effectors and are a highly attractive alternative to Cas9 Nucleases for engineering mammalian genomes as they reveal higher specificity via trans-cleaving non specific ssDNA, which minimizes off-target effects. They are also smaller in size, which makes them more suitable for potential gene therapy approaches, and they tolerate a wide temperature range, which is ideal for biomedical gene detection techniques.
Nucleus Biotech is pleased to offer Trialtus Biosciences´ recombinant AapCas12b originating from Alicyclobacillus acidiphilus (see Teng et al., Nature, Cell Discovery volume 4, Article number: 63 (2018) for single and multiplex genome editing, gene activation, and generation of gene mutants with CRISPR technology and for CRISPR-mediated in vitro applications.
TriAltus’ highly pure recombinant AapCas12b protein combines with suitable guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables the inactive nuclease to be guided to the specific double stranded DNA (dsDNA) site. Once guided to the specific cleavage site, AapCas12b becomes activated to specifically and precisely cleave the dsDNA and to indiscriminately trans-cleave single stranded DNA (ssDNA) nearby.
Trialtus Biosciences´ protein is purified tag-free by their proprietary CL7 tag CLiM technology, which ensures extremely high purity and activity:
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