NGS Reagents for Pooled sgRNA Libraries

NGS Reagents for Pooled sgRNA Libraries are tools to amplify and sequence sgRNAs, after running screens with pooled lentiviral libraries. For CRISPR screens, the frequency of each integrated sgRNA is assessed by next-generation sequencing (NGS) to determine which ones are enriched or depleted relative to the original libraries or control samples.
Nucleus Biotech provides NGS Prep Kits for various libraries to meet your needs. Each contains the appropriate primers for particular library/vector combinations to generate sequencing-ready samples. Simply select the Prep Kit for your screen and order.

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NGS Prep Kit for sgRNA Libraries in pRSG16/17-including Alignment Software

Cat.#: LNGS-120

Qty.: Quantity: Kit (48x 50ug preps)

Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the pRSG16 and pRSG17 vectors (e.g. CRISPR Human, Mouse, Chicken, or Pig Genome Knockout Library) prov...

Supplementary Primer Set for LNGS-120 (12 Primers)

Cat.#: LNGS-120-SP

Qty.: Quantity: 48 Reactions

The NGS Prep Supplementary Primer Set provides an additional 12 indexing primers for use with the LNGS NGS Prep kit LNGS-120. When used with the corresponding N...

NGS Prep Kit for Tet-sgRNA Libraries in pRSGT16/17 -including Alignment Software

Cat.#: LNGS-130

Qty.: Quantity: Kit (48x 50ug preps)

Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the pRSGT16 and pRSGT17 vectors provides a protocol and reagents for the PCR amplification from genomi...

Supplementary Primer Set for LNGS-130 (12 Primers)

Cat.#: LNGS-130-SP

Qty.: Quantity: 48 Reactions

The NGS Prep Supplementary Primer Set provides an additional 12 indexing primers for use with the LNGS NGS Prep kit LNGS-130. When used with the corresponding N...

NGS Prep Kit for sgRNA Libraries in pRSGScribe-including Alignment Software

Cat.#: LNGS-360

Qty.: Quantity: Kit (48x 50ug preps)

Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the pRSGScribe vector provides a protocol and reagents for the PCR amplification from genomic DNA and ...

Supplementary Primer Set for LNGS-360 (12 Primers)

Cat.#: LNGS-360-SP

Qty.: Quantity: 48 Reactions

The NGS Prep Supplementary Primer Set provides an additional 12 indexing primers for use with the LNGS NGS Prep kit LNGS-360. When used with the corresponding N...

NGS Prep Kit for Dual-sgRNA Libraries in pRSL10-including Alignment Software

Cat.#: LNGS-400

Qty.: Quantity: Kit (48x 50ug preps)

Cellecta’s NGS Prep Kit for Dual sgRNA Libraries cloned into the pRSL10 vector provides a protocol and reagents for the PCR amplification from genomic DNA and...

Supplementary Primer Set for LNGS-400 (12 Primers)

Cat.#: LNGS-400-SP

Qty.: Quantity: 48 Reactions

The NGS Prep Supplementary Primer Set provides an additional 12 indexing primers for use with the LNGS NGS Prep kit LNGS-400. When used with the corresponding N...

NGS Prep Kit for sgRNA Libraries in LentiGuide

Cat.#: LNGS-900

Qty.: Quantity: Kit (48x 50ug preps)

Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the LentiGuide vector (e.g. GeCKO, Brunello) provides a protocol and reagents for the PCR amplificatio...

Supplementary Primer Set for LNGS-900 (12 Primers)

Cat.#: LNGS-900-SP

Qty.: Quantity: 48 Reactions

The NGS Prep Supplementary Primer Set provides an additional 12 indexing primers for use with the LNGS NGS Prep kit LNGS-900. When used with the corresponding N...

NGS Prep Kit for sgRNA Libraries in LentiCRISPRv2

Cat.#: LNGS-905

Qty.: Quantity: Kit (48x 50ug preps)

Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the LentiCRISPRV2 vector (e.g. GeCKO, Brunello Kinome) provides a protocol and reagents for the PCR am...

Supplementary Primer Set for LNGS-905 (12 Primers)

Cat.#: LNGS-905-SP

Qty.: Quantity: 48 Reactions

The NGS Prep Supplementary Primer Set provides an additional 12 indexing primers for use with the LNGS NGS Prep kit LNGS-905. When used with the corresponding N...

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