Cell System for TCR-Antigen Identification and Characterization
Identify and analyze T-Cell Receptor (TCR) – Antigen Interactions
30% introductory discount
Nucleus Biotech offers Cellecta´s engineered reporter cell lines and controls, to run the entire TCR-Antigen identification and characterization workflow in your lab, without requiring specialized equipment. Any academic, biotech, and pharma lab that has access to a flow sorter can now identify and analyze T-Cell Receptor (TCR) – Antigen interactions at ease.
Genes encoding TCR chains can be introduced and expressed in Jurkat TCR-Reporter Cells, which enable TCR-Epitope-MHC interactions to activate expression of GFP. In parallel, appropriate Human Leukocyte Antigens (HLAs) can be expressed in K562 Antigen-Presenting cells (APC). Antigens may then be loaded onto or co-expressed by the MHC-APC Cells to activate the Jurkat Reporter Cells with the appropriate TCR.
Scientific Poster Presentation
Complete Workflow for TCR-Epitope Discovery
We are pleased to offer an end-to-end pipeline for the discovery of functional TCR-epitope interactions. Starting from quantitative adaptive immune receptor (AIR) bulk profiling to identify activated clonotypes in tumor/blood samples, followed by single-cell AIR sequencing to obtain TCR α/β chain pairs. You then can use this information to clone and reconstruct TCR candidates in Jurkat Reporter Cells and profile peptide antigens against it using K562 APC Cells or multimer-conjugated peptide fluorophore assay.
Cell Lines Engineered to Assess Antigen-Receptor Interactions 30% introductory discount
Jurkat TCR Knockout and Jurkat NFAT-GFP Reporter Cell Lines: The genes encoding the TCR α and β chains have been knocked out in this clonal derivative of the Jurkat E6.1 line. In addition, Cellecta has also created a clonal reporter cell line by introducing a stable NFAT-responsive GFP reporter into the TCR knockout cells so that activation of expressed TCRs can be easily detected by fluorescence. The Jurkat reporter line also expresses CD8, which is important to stabilize TCR-MHC interaction.
K562 HLA-A-02:01 Antigen-Presenting Cell (APC) Lines: K562 cells have been engineered with the genes encoding the HLA-A-02:01 complex so that cells can effectively present peptide antigens to activate T cells with the appropriate TCRs. These K562 APC Cells are available with Puro or BFP expression markers for co-culturing with TCR-expressing Jurkat Reporter Cells.
Control Cell Lines: Also available are peptide-specific Jurkat TCR Reporter Lines expressing CMV, EBV, or FLU TCR´s, to serve as control cell lines with known peptide constructs. As a co-culturing control cell line CMV peptide expressing K562 HLA-A-02:01 cells are offered.
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