Single Cell TCR and BCR Sequencing - Chain Pairing Assays

Single-Cell TCR & BCR Sequencing for Chain Pairing Detection and Single Immune Cell Phenotyping

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Single-cell adaptive immune receptor repertoire sequencing (scAIRR-seq) enables to access the nucleotide sequences of paired receptor chains from T-cell receptors (TCR) or B-cell receptors (BCR ), which is crucial to explore the diversity of immune cells in depth and is necessary to clone functional TCRs resp. antibodies for antigen discovery studies and the development of T Cell- or antibody-based therapies.

While the Bulk DriverMap™ AIR RNA/DNA Immune Repertoire Profiling Assays allows quantitative profiling of abundant clonotypes, the scAIRR-seq assays are designed to get additional full-length chain pairing information and phenotypes of T- or B-cell clonotypes.

Nucleus Biotech is pleased to introduce Cellecta´s DriverMap Single-Cell AIRR-seq kits (see below) and services for identification of paired full-length TCR or BCR sequences as well as cell phenotyping by biomarker expression profiling at single-cell resolution.

• Efficient single-cell immune profiling strategy using sorted cells in 96-well plate

• Cost-effective without the need for any microfluidics- or split pool-based single-cell technologies

• Multiplex RT-PCR combined with NGS enables the sequencing of full-length TCR or BCR chains at single-cell resolution along with the gene expression profiling of crucial 36 T- or 30 B-cell markers

DriverMap scAIR Workflow:

The following chart demonstrates the use of well/cell-specific barcodes, and of gene specific primers which are highly specific to TCRs/BCRs and T-Cell/B-Cell phenotyping markers and incorporate Validation Barcodes (VBC), to  generate 96 single-cell AIRR-seq libraries by a highly efficient pooled Multiplex RT-PCR – based NGS library preparation protocol:

Outline of DriverMap scAIR assays for paired TCR/BCR chain analysis and targeted expression profiling of T/B cell marker genes. All Rev-GSPs have cell/well-specific barcodes, TCR/BCR Rev-GSPs have validation barcodes (VBCs) to facilitate quantitative analysis of paired receptor chains.