Lentiviral Packaging Plasmids

While lentiviral vectors are popular gene delivery tools, generating viral particles can be challenging. To overcome these hurdles Cellecta offers ready-to-use Packaging Plasmid Mixes providing all the necessary structural, regulatory, and replication proteins required to efficiently produce pseudotyped packaged lentiviral expression constructs for high-efficiency transduction. The resulting pseudotyped lentiviral particles can infect a variety of mammalian or non-mammalian, dividing or non-dividing cells.
Nucleus Biotech provides Ready-to-use Packaging Mixes that will produce high titer lentivirus with an easy to use protocol, and are compatible with most commercially-available second- and third generation lentiviral vectors.

Two different optimized Packaging Mixes are offered:

• The most commonly used two plasmid mix, which contains pMD2.G for VSV-G envelope production, and the HIV packaging plasmid psPAX2, pre-mixed in an optimized ratio

• A three plasmid mix, which is required in some labs, and contains pMD2.G for VSV-G envelope production as well as the two HIV packaging plasmids pGAG and pREV, pre-mixed in an optimized ratio

All 4 plasmids mentioned above are also available separately as individual plasmids for producing your own packaging plasmid mix in your laboratory. This allows the flexibility to use different packaging plasmid ratios, for example, to maximize titer in your cell line of interest. It also allows for the use of alternative envelope plasmids resp. other packaging plasmids.