BioDynami´s DNA Fragmentation Enzyme Mixes were developed for random fragmentation of genomic DNA. Enzyme-based fragmentation of DNA is easy and effective in comparison to mechanical DNA shearing methods and does not require expensive equipment.
The resulting DNA fragment size is inversely correlated with the enzyme incubation time.
The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS), as BioDynami´s Fragmentation Enzyme Mix does not generate any detectable sequencing bias, and sequence coverage is also consistent between enzymatic and mechanical fragmentation.
BioDynami´s DNA Fragmentation Enzyme Mixes are also available with additional A-Tailing resp. Blunting enzymes to enable AT or blunt-end cloning of the resulting fragments.