Magnetic Beads for DNA Normalization

Bead-based DNA Normalization

Consistent DNA quantity normalization of various sources in excellent dynamic range
Easy and efficient protocol – no centrifugation, no filtering
No more fluorescent quantitation needed
Cost and Time Saving
Removal of unwanted components and impurities
Elution in as low as 10-20 µl without yield loss
Ideal for High Throughput Sample Processing

DNA Normalization Magnetic Beads were developed to normalize NGS libraries, PCR fragments, sheared genomic DNA, and genomic DNA based on BioDynami´s magnetic bead technology.

During bead-based normalization, any DNA samples of varying concentrations are bound to BioDynami´s magnetic DNA normalization beads characterized by a defined limited DNA binding capacity. Bound DNAs are separated from excess unbound DNA and are subsequently eluted off the beads at approximately the same concentration for each sample.

Specifications of DNA Normalization Magnetic Beads

Cat.#	40071/40072	40073	40075	40077	40079
NGS Library Input	√	√	√	√	√
PCR Input	√	√	√	√	√
Sheared gDNA Input	√	√	√	√	√
gDNA Input	√
Input Range	NGS, PCR: 300-2000ng gDNA: 500-5000ng	150-1200 ng	50-300 ng	15-100 ng	5-30 ng
Normalized Range	NGS, PCR: 100-180ng gDNA: 150-270ng	100-320 ng	25-60 ng	5-15 ng	1.5 -4.5 ng
Elution Volume	>20 μl	>10 μl	>10 μl	>10 μl	>10 μl

BioDynami kits 40072S and 40072L additionally include special recovery beads for the recovery of unbound excess DNA during DNA normalization.

Traditional fluorescent methods of normalization require the setup of a standard curve from known concentrations, compare with samples, aliquot adjusted volume for each sample, and add adjusted volume of elution buffer to match the total volume for each sample. The whole procedure needs extensive efforts, expensive reagents, and has a long processing time. In contrast, the beads-based protocol is simple, fast, centrifugation free, and filtration free. Additional DNA quantification and dilution is not necessary after normalization with magnetic beads. Labor, time, and reagents are all saved by the use of DNA normalization beads.

Applications of BioDynami´s Magnetic DNA Normalization Beads:

NGS Library Normalization

Magnetic bead-based NGS library normalization provides significant cost and time savings especially when processing many samples.

Normalization of NGS Libraries: Input NGS library amounts and normalized NGS library amounts

This efficient, time-saving alternative bypasses time-consuming and costly library quantitation measurements using fluorescence and/or fragment analyzer instruments. Moreover, manual pipetting steps including dilutions before loading NGS libraries onto the sequencer are no longer necessary.

Normalization of Genomic DNA and of PCR Products

Varying amounts of genomic DNA (including sheared genomic DNA fragments in different samples) as well as of PCR Products can easily and conveniently be normalized to uniform DNA concentrations by BioDynami´s Magnetic DNA Normalization Beads.

Normalization of Genomic DNA: Input Genomic DNA amounts and normalized Genomic DNA amounts

Normalization of PCR Products: Input PCR Products DNA amounts and normalized PCR Products DNA amounts

BioDynami´s unique Magnetic Beads Technology

BioDynami´s magnetic beads are based on SPRI (Solid Phase Reversible Immobilization) and consist of paramagnetic particles coated with carboxyl groups that reversibly bind nucleic acids or paramagnetic particles coated with a proprietary chemistry enabling binding to small nucleic acids -technology background information can be found in the publication from Ribeiro et al. (ACS Omega, Jan 21, 2026)