LNP-based RNA Transfection

LNP-based Transfection of mRNA, siRNA, circRNA, and saRNA

Easy RNA Encapsulation into LNPs (Lipid Nano Particles) for high efficiency in vitro cell transfections as well as systemic or localized in vivo delivery

Levostar´s NeoLNP: Efficient RNA encapsulation in 1 minute

NeoLNP was developed by Levostar Pharmaceutical based on an advanced lipid material library platform and enables RNA delivery on a whole new level of precision and efficiency. NeoLNP encapsulates RNA into LNPs by a simple one minute mixing and utilizes novel biodegradable lipids enabling a unique transmembrane transport and endosomal escape mechanism for RNA delivery into the cell at unmatched efficiency. NeoLNP is suitable for transfecting mRNA, siRNA, circRNA, saRNA, and other types of RNA.

The reagent is offered in kit formats for in vitro cell transfection, and for in vivo systemic or localized delivery by injection. You can also take advantage of our LNP Encapsulation Services.

NeoLNP in vitro Cell RNA Transfection

▪ Broad Cell Compatibility: Successfully validated in a large variety of cell types, including HeLa, A549, Capan-2, HEK293T, HCT116, L929 and many more

▪ Special kits optimized for human and mouse immune cell lines and primary immune cells

▪ Versatility: For transfecting mRNA, siRNA, circRNA, saRNA, and other RNA types

▪ High Transfection Efficiency: Enabled by its unique biodegradable lipid composition built for optimal transmembrane transport and endosomal RNA escape, transfection efficiencies exceed 90% for most common cell lines, and 80% for immune cells, which surpasses industry standards by far

▪ Extremely Low Cytotoxicity: Utilizes biodegradable materials, free of ethanol, with minimal impact on cells, reducing experimental bias

NeoLNP Cell RNA Transfection Data

NeoLNP mRNA transfection of cell lines

HEK293T-, HeLa-, and HCT116- cells were transfected in a 24-well plate with eGFP mRNA using NeoLNP versus other brands, followed by fluorescence imaging after 24 hours.

NeoLNP siRNA transfection of cell lines

Cellular siRNA uptake efficiency using NeoLNP was compared with RNAimax by transfecting HEK293T-, HeLa-, HCT116-, and L929- cells with 30nM Cy3-labeled siRNA per well, followed by fluorescence imaging after 6 hours.

NeoLNP circRNA transfection of cell lines

HEK293T- and HCT116- cells were transfected in a 96-well plate with 100 ng eGFP circRNA using NeoLNP versus jetMESSENGER, followed by fluorescence imaging after 24 hours.

NeoLNP saRNA transfection of cell lines

HEK293T- and HCT116- cells were transfected in a 96-well plate with 100 ng eGFP saRNA using NeoLNP, followed by fluorescence imaging after 24 hours.

NeoLNP in vivo Systemic or Localized RNA Delivery

▪ High Efficiency: Enabled by its unique biodegradable composition built for optimal transmembrane transport and endosomal RNA escape, NeoLNP maintains stable high transfection levels regardless of injection method

▪ Flexible: Novel biodegradable lipids allow truly systemic delivery reaching various organs as well as topical RNA delivery, e.g. to the lung, intratumoral etc.

▪ Versatility: For delivery of mRNA and siRNA

▪ User-Friendly: Optimized delivery solution which enables easy, rapid, and efficient RNA encapsulation without the need for microfluidic systems, simplifying experiments

NeoLNP in vivo RNA Transfection Data

 Intravenous Injection (IV) – Targeting Liver/Spleen

Intramuscular Injection (IM) – Vaccines

Intraperitoneal Injection (IP) – Multi-Organ/Tissue RNA Delivery