LNP Encapsulation Services

Lipid Nano Particles – Next Generation RNA Delivery

Lipid Nanoparticles (LNPs) utilize lipid molecular self-assembly to create nano-scale encapsulation systems. This technology significantly enhances drug stability and targeting efficacy.

Levostar´s NeoLNP is based on an advanced lipid material library platform and enables RNA delivery on a whole new level of precision and efficiency. NeoLNP encapsulates RNA into LNPs by a simple one minute mixing and utilizes novel biodegradable lipids enabling a unique transmembrane transport and endosomal escape mechanism for in vitro and in vivo RNA delivery at unmatched efficiency. NeoLNP is suitable for transfecting mRNA, siRNA, circRNA, saRNA, and other types of RNA.

Levostar´s NeoLNP: Efficient RNA encapsulation in 1 minute

Levostar LNP Encapsulation Service Advantages

· Fully integrated technology platform covering gene synthesis, plasmid preparation, mRNA-, circRNA– or saRNA– synthesis (or premade mRNA, circRNA, saRNA), and LNP encapsulation

· Superior Nanoparticle Characterization：QC analytics ensures precision and reliability

· Flexible Scaling Capacity：Manufacturing from 1 mg to 100 mg

Service Details

 Encapsulation Parameters

QC Specifications
Scale：1 mg -100 mg pH：6.0-8.0
Payload Capacity：100 nt~20 kb mRNA Size Distribution：40-180 nm
Cationic Lipids：SM102 / ALC-0315 PDI：≤ 0.5
Turnaround Time：~2 weeks Encapsulation Efficiency：≥ 85%
Shipping Condition：In aliquots on dry ice. Single freeze/thaw cycle RNA Content：0.1-0.3 mg/ml
Optional Test Annotation：Zeta、Empty Vesicle Ratio

Antibody-conjugated LNPs

Utilizing antibody-conjugated LNPs enables improved targeting and transfection efficiencies (e.g. for CAR-T generation in cell therapy approaches) as shown here for CD3-antibody conjugated LNPs boosting GFP mRNA delivery to Jurkat cells:

LNP GFP mRNA Delivery to Jurkat Cells

LNP-CD3ab GFP mRNA Delivery to Jurkat Cells

Levostar is offering customized LNP conjugation to an antibody of your choice including analysis of key parameters such as particle size distribution, and antibody positivity rate. Please contact us for a quotation.

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