Synthetic mRNAs for iPSC Reprogramming

iPSC Reprogramming mRNAs for in vitro transfection and in vivo delivery by injection

Safe footprint-free generation of induced pluripotent stem cells
No genomic integration
Lowered immunogenicity and oncogenicity compared to viral approaches
High efficiency IPSC reprogramming

mRNAs encoding the Yamanaka factors KLF4, c-Myc, OCT4, and SOX2

Cellular reprogramming factors are key transcriptional regulators that drive the conversion of differentiated somatic cells toward a pluripotent state. Among the best-established cellular reprogramming factors are the Yamanaka factors KLF4, c-MYC, OCT4, and SOX2, whose coordinated activity induces extensive transcriptional and epigenetic cell remodeling during iPSC generation.

Synthetic mRNAs provide a particularly well-suited approach for transient, non-integrating delivery of the reprogramming factors at highest efficiencies. Following cellular uptake, mRNA is directly translated into the corresponding transcription factors without introducing exogenous DNA into the genome, enabling controlled cellular reprogramming.

mRNA-based reprogramming allows safe and footprint-free iPSC generation:

No Genomic Integration: Synthetic mRNAs translate in the cytoplasm without entering the nucleus or altering host DNA.

High Safety Profile: Transient expression limits oncogenic risks and lowers immunogenicity compared to viral vectors.

mRNAs encoding additional proteins supporting IPSC reprogramming

In addition to the Yamanaka factor mRNAs, Nucleus Biotech is pleased to offer mRNAs encoding the following IPSC generation-supporting proteins:

B18R – IFN Inhibitor for suppression of IFN-induced cell differentiation

GLIS1 – Chromatin accessibility modulator enhancing Yamanaka factors

LIN28A – let-7 mRNA modulator enhancing translation of core stemness factors

NANOG – Cooperates with OCT4 and SOX2 to enhance stem cell renewal and suppress cell differentiation

NR5A2 – Nuclear receptor that regulates pluripotency gene networks, important for stem cell maintenance

Nucleus Biotech presents Levostar´s premade mRNAs encoding iPSC reprogramming factors, featuring enhanced stability, prolonged protein expression, and scalable manufacturing capabilities.

Comprehensive mRNA Catalog and mRNA Synthesis Services

In addition, Nucleus Biotech is pleased to offer premade synthetic mRNAs encoding fluorescent proteins and other controls, immune cell receptors, immune checkpoint molecules, tumor-associated antigens, cytokines and growth factors, as well as nucleases, transposases, & recombinases for genome engineering approaches.

Find out more about our entire collection of premade mRNAs

In case you can´t find your mRNA of interest, you can use our mRNA synthesis services.

We are also offering Levostar´s NeoLNP product line for easy and highly efficient mRNA encapsulation and LNP-based in vitro mRNA transfection as well as in vivo mRNA delivery by injection.

Levostar mRNAs are designed for optimal expression results:

• Enhanced polyA(+) Stability – for preventing RNA degradation

• Cap1 Structure – for optimal ribosome recruiting

• Carefully selected UTR Regions – to enhance translation initiation

N1-Methylpseudouridine Modification

For enhanced mRNA stability and decreased anti-mRNA immune response Levostar´s catalog mRNAs are in vitro transcribed using N1-Methylpseudouridine (N1-Me-Ψ, N1mpU) by default. If you prefer unmodified mRNA or you require a different modification, please contact us.

monoPlasmid DNA Technology

Levostar mRNAs are manufactured by their proprietary monoPlasmid Technology enabling high In Vitro Transcription (IVT) yields and outstanding polyA(+) stability:

monoPlasmid: IVT Plasmids with high monomer ratio

Due to the presence of the polyA tail, mRNA plasmids are highly prone to inter-plasmid recombination, resulting in the formation of large plasmids as dimers or multimers. Multimer formation significantly reduces plasmid yield. LevoStar´s monoPlasmid platform incorporates plasmid elements as well as a proprietary bacterial strain, together preventing multimer formation, which ensures a plasmid monomer supercoiling rate of over 95% while also substantially increasing plasmid yield.

Common mRNA plasmids show a high ratio of multimers

monoPlasmid mRNA plasmids show a high ratio of monomers

monoPlasmid: IVT Plasmids with stabilized polyA(+) tails

Due to its low free energy, the polyA sequence in mRNA plasmids is prone to breakage within E. coli, leading to partial or complete loss of the polyA tail during plasmid repair. Such polyA-deficient mRNA plasmids cannot be used to produce functional mRNA. Levostar´s monoPlasmid platform incorporates a specialized sequence that significantly reduces the likelihood of such loss.

Sanger Sequencing demonstrates partial loss of the polyA tail in common mRNA plasmids

Sanger Sequencing demonstrates complete polyA tail presence in monoPlasmid mRNA plasmids

Quality Standards: All premade mRNAs are tested for high purity, and are characterized by low endotoxin, low residual dsRNA & plasmid template DNA, high capping efficiency, and proper polyA(+)-length.

We are pleased to offer premade mRNAs encoding iPSC reprogramming factors: