2′-O-Methyltransferase (2OM) (High Efficiency, High Purity)

Description

Highly purified and extremely active 2′-O-Methyltransferase (2OM)

For extremely efficient conversion of Cap0-capped mRNA to Cap1-capped mRNA

• High purity-grade recombinant enzyme for mRNA manufacturing workflows involving post-transcriptional mRNA capping
• Using S-adenosyl methionine (SAM) as the methyl donor, 2OM adds a methyl group to the 2′-O position of the first nucleotide immediately adjacent to the Cap0 mRNA cap structure at the 5′ end
• Converts Cap0-capped mRNA extremely efficiently to Cap1-capped mRNA
  

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  Cap1 (m7GpppNm), common in higher eukaryotes such as mammals, has been shown to be important for evading the intracellular immune system, and to increase the stability and translational activity of the mRNA. .       Biori´s mRNA Cap 2´-O-Methyltransferase (originally deriving from Vaccinia) is a multi-step purified recombinant highly efficient enzyme preparation manufactured by Biori Biotech to GMP standards. It modifies the Cap0 cap m7Gppp into the Cap1 cap structure m7GpppNm using S-adenosylmethionine (SAM) as the methyl donor,  and adding a methyl group to the 2′-O position of the first nucleotide immediately adjacent to the Cap0 cap structure at the 5′ end of the RNA, thereby generating an mRNA bearing the Cap1 structure.

Contents:

1 ml  mRNA Cap 2´-O-Methyltransferase (50 U/ul), 50000 Units, sufficient for 1000 Capping Reactions (based on the input of 50 U for 50 pmol denatured Cap0 mRNA)

2 ml  10x Capping Buffer

0.2  ml    32mM SAM (S-adenosylmethionine)