Adenine Base Editor ABE8e mRNA

Description

Ready-to-use synthetic Adenine Base Editor ABE8e mRNA

For in vitro mRNA transfection and in vivo mRNA delivery by injection

Encodes the adenine base editor ABE8e, an engineered CRISPR-Cas9 variant consisting of nSpCas9 (D10A) Nickase from Streptococcus pyogenes fused to the TadA-8e adenine deaminase, that catalyzes A•T to G•C conversions with enhanced efficiency and precision for therapeutic genome editing. This synthetic mRNA enables transient, dose-controlled delivery of ABE8e for research in:
Correcting pathogenic SNVs (e.g., Tyr402Cys in C1-INH for hereditary angioedema), developing ex vivo cell therapies (e.g., base-edited CAR-T cells), and studying epigenetic modulation via targeted adenine methylation

Manufactured by Levostar´s monoPlasmid technology

Designed for optimal expression results:

• Enhanced polyA(+) Stability – for preventing RNA degradation

• Cap1 Structure – for optimal ribosome recruiting

• Carefully selected UTR Regions – to enhance translation initiation

N1-Methylpseudouridine Modification

For enhanced mRNA stability and decreased anti-mRNA immune response Levostar´s catalog mRNAs are in vitro transcribed using N1-Methylpseudouridine (N1-Me-Ψ, N1mpU) by default. If you prefer unmodified mRNA or you require a different modification, please contact us.

Quality Standards: Tested for high purity, low endotoxin,  low residual dsRNA & plasmid template DNA, high capping efficiency, and proper polyA(+)-length.

Contents:  1 mg mRNA, lyophilized          – also available in solution (1 mg/ml), please inquire

Shipping and Storage:   Lyophilized mRNA shipped at room temperature (≤ 25°C). Store at –80°C upon dissolving.

For other mRNA quantities than 1 mg including bulk offers please contact us for pricing

mRNA may also be ordered already encapsulated in Lipid Nanoparticles for high efficiency mRNA delivery – please contact us for a quotation.

For mRNA encapsulation in your lab and subsequent in vitro mRNA transfection or in vivo mRNA delivery we offer Levostar´s portfolio of NeoLNP Kits.