Cas12a Protein (Lachnospiraceae bacterium), LbCas12a (LbCpf1), with 2x C-terminal NLS

Description

Recombinant Cas12a Protein for High Fidelity CRISPR Applications: LbCas12a (LbCpf1)

• Recombinant LbCas12a (LbCpf1) for forming RNP complexes with guide-RNA
• Highly stable and free of RNAses
• Highly purified and extremely active (staggered double strand DNA cleavage)
• Trans ssDNA cleavage activity ensures high fidelity
• Bulk Pricing available – please contact us for a quote

TriAltus Bio´s highly purified recombinant Lachnospiraceae bacterium Cas12a (LbCas12a, LbCpf1) protein (>90% purity) with 2 C-terminal nuclear localization signals (NLS) is used for CRISPR-based high fidelity gene editing technologies.  The enzyme was expressed in E. coli and purified by TriaAltus Bio´s proprietary CL7 Tag/CLiM technology and is delivered tag-free.

LbCas12a protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables the inactive nuclease to be guided to the specific double stranded DNA (dsDNA) site. Once guided to the specific cleavage site, LbCas12a becomes activated to specifically and precisely cleave the dsDNA and to indiscriminately trans-cleave single stranded DNA (ssDNA) nearby.

TriAltus’ highly pure, tag-free LbCas12a is ideal for genome editing, CRISPR diagnostics, and multiplexed gene targeting. This recombinant enzyme, from Lachnospiraceae bacterium, is expressed in E. coli and includes two C-terminal nuclear localization signals (NLS). LbCas12a is a Class 2, Type V-A CRISPR nuclease that cuts DNA at thymine-rich PAM sites (5’-TTTV), creating staggered 5′ overhangs for precise gene insertion. It also processes its own guide RNAs, making it well-suited for editing multiple genes at once. With lower off-target activity than Cas9 and strong trans-cleavage activity, LbCas12a supports both high-fidelity genome engineering and sensitive CRISPR-based detection.

LbCas12a shows a very good activity also at lower temperatures, and has been widely used for plant genome engineering projects.

SPECIFICATIONS

PURITY | >90% (SDS-PAGE gel analysis)

ENZYME SOURCE | Lachnospiraceae sp. Cas12a is expressed in E. coli with two C-terminal nuclear localization signals (NLS), purified by CLiM-Technology, and delivered tag-free

QUANTITY | 500 ug

STORAGE/SHIPPING CONCENTRATION | 5 ug/ul

STORAGE BUFFER | 10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol

RECOMMENDED STORAGE CONDITIONS | -80°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.