Description
LbuCas13a Kit for Precision RNA Target Sequence Detection Technologies
- Recombinant highly purified and active LbuCas13a and a custom guide-RNA specifically designed to detect your RNA substrate for RNP complex forming
- Optimized Reaction Buffer for precise RNA target detection and trans ssRNA cleavage
- ssRNA reporter which generates fluorescence signal upon trans ssRNA cleavage
- Bulk Pricing available – please contact us for a quote
KIT DESCRIPTION:
SignalChem´s highly active LbuCas13a protein (purified with a his tag) combines with your target specific Cas13a guide-RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables the inactive nuclease to be guided to the specific RNA site. Once guided to the specific cleavage site, LbuCas13a becomes activated to specifically and precisely cleave the RNA and to indiscriminately trans-cleave single stranded RNA (ssRNA) nearby. A short single-stranded RNA labeled with a fluorophore and a quencher on the two termini is used as a reporter to generate a detectable fluorescence signal for highly precise detection of the guide RNA-targeted RNA sequence. The highly precise detection system can be combined with pre-amplification procedures including isothermal amplifications such as RPA (Recombinase Polymerase Amplification) and LAMP, to achieve one-pot reaction systems, suitable for field-testing, and offering many advantages over qPCR-based detection. The use of various Cas13 proteins with different substrates in one assay enables multiplexed CRISPR-based target RNA detection, which might be combined with target DNA detection by using Cas12 protein/s.
KIT CONTENTS:
100 ug LbuCas13a (Leptotrichia buccalis Cas13a) recombinant protein
2 x 1ml Cas13 Reaction Buffer II (10x)
2 nmol custom Cas13 guide-gRNA – let us know your RNA target
10 nmol Cas13 Fluorescence ssRNA Reporter (FAM-6 labeled; absorption at 495 nm/emission at 517 nm, other labels available on request)
- Kit can also be ordered without custom guide-RNA or without ssRNA reporter – please contact us for a quote
- All kit components can also be ordered separately – please contact us for a quote