Description
Recombinant Cas9 Protein for CRISPR Applications – SpCas9, glycerol free formulation
- Recombinant wt SpCas9 for forming RNP complexes with guide-RNA
- Highly stable and free of RNAses
- Highly purified and extremely active (blunt end ds DNA breaks)
- NLS for delivery into the nucleus and high efficiency genome editing
- Suitable for RNP-transfection of primary cell cultures and high-throughput use of instruments with sensitive fluidics due to glycerol absence
- Bulk Pricing Available – please contact us for a quote
CasRNPs are an improvement on plasmid transfections because the Cas protein is cleared from the system within 24 hours, decreasing off-target effects. The method also saves time, as the active complex is what’s being delivered straight to the nucleus of the cell. Editing efficiency is correlated with Cas protein purity, so it’s crucial that high quality Cas9 such as the highly purified SignalChem Dx Cas9 protein is used.
SignalChem’s highly purified recombinant glycerol free Streptococcus pyogenes Cas9 (wt) protein (>90% purity) with N-terminal nuclear localization signal (NLS) is used for genome editing with CRISPR technology and CRISPR-mediated in vitro applications. The enzyme was expressed in E. coli with His tag, which was used during the purification process. SpCas9 protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables the inactive nuclease to be guided to the specific double stranded DNA (dsDNA) site. Once guided to the specific cleavage site, SpCas9 becomes activated to specifically and precisely cleave both DNA strands creating blunt ends.
SPECIFICATIONS
PURITY | >90% (SDS-PAGE gel analysis)
ENZYME SOURCE | Streptococcus pyogenes Cas9 is expressed in E. coli with N-terminal nuclear localization signal (NLS), and is purified using a His tag.
QUANTITY | 100 ug
STORAGE/SHIPPING CONCENTRATION | 0.1 ug/ul
STORAGE BUFFER | 50mM sodium phosphate pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 5% trehalose
RECOMMENDED STORAGE CONDITIONS | -80°C
EXPIRATION | 12 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.