Description
Recombinant Cas9 Protein for CRISPR Applications: SpCas9
- Recombinant wt SpCas9 for forming RNP complexes with guide-RNA
- Highly stable and free of RNAses
- Highly purified and extremely active (blunt end ds DNA breaks)
- No NLS – suitable for targeting DNA outside of the nucleus, e.g. viral DNA
SignalChem’s highly purified recombinant Streptococcus pyogenes Cas9 (wt) protein (>80% purity) without nuclear localization signal (NLS) is used for CRISPR applications targeting DNA outside the nucleus (e.g. viral DNA) and for CRISPR-mediated in vitro applications. The enzyme was expressed in E. coli with His tag, which was used during the purification process. SpCas9 protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables the inactive nuclease to be guided to the specific double stranded DNA (dsDNA) site. Once guided to the specific cleavage site, SpCas9 becomes activated to specifically and precisely cleave both DNA strands creating blunt ends.
SPECIFICATIONS
PURITY | >80% (SDS-PAGE gel analysis)
ENZYME SOURCE | Streptococcus pyogenes Cas9 is expressed in E. coli without nuclear localization signal (NLS), and is purified using a His tag.
QUANTITY | 100 ug
STORAGE/SHIPPING CONCENTRATION | 0.5 ug/ul
STORAGE BUFFER | 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol
RECOMMENDED STORAGE CONDITIONS | -80°C
EXPIRATION | 12 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.