Categories

Cas9(D10A, H840A) Protein (S. pyogenes), dCas9, with N-terminal NLS

975,00 

Quantity: 1000 ug

    Please contact us for a quotation

     


    Description

    Recombinant Dead Cas9 Protein for CRISPR-based transcriptional and epigenetic genome modulations, visualization of genomic loci, and disease diagnosis: SpCas9(D10A, H840A)

    • Recombinant SpCas9(D10A, H840A) for forming RNP complexes with guide-RNA
    • Highly stable and free of RNAses
    • Highly purified and extremely efficient target DNA Binding (no nucleolytic activity)
    • NLS for delivery into the nucleus

    SignalChem’s highly purified recombinant Streptococcus pyogenes Cas9(D10A, H840A) protein (>70% purity) with N-terminal nuclear localization signal (NLS) is used for DNA binding studies including transcriptional repression and activation (CRISPRi/CRISPRa), epigenomic remodeling, visualization of genomic loci, and disease diagnosis applications, as well as for CRISPR in vitro applications.  The enzyme was expressed in E. coli with His tag, which was used during the purification process. SpCas9(D10A, H840A) protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex guided to the specific target site, and does not have any nucleolytic activity.

    SPECIFICATIONS

    PURITY | >70% (SDS-PAGE gel analysis)

    ENZYME SOURCE | Streptococcus pyogenes Cas9(D10A, H840A) is expressed in E. coli with N-terminal nuclear localization signal (NLS), and is purified using a His tag.

    QUANTITY | 1000 ug

    STORAGE/SHIPPING CONCENTRATION | 0.2 ug/ul

    STORAGE BUFFER | 50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol

    RECOMMENDED STORAGE CONDITIONS | -80°C

    EXPIRATION | 12 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.

    Shipping Conditions: Dry Ice
    Storage Temperature: -80C

    You may also like…