CD73 (NT5E) mRNA

Description

Ready-to-use CD73 (NT5E) mRNA

For in vitro mRNA transfection and in vivo mRNA delivery by injection

Encodes the ecto-5′-nucleotidase CD73 (NT5E), a GPI-anchored cell surface enzyme that converts extracellular AMP to immunosuppressive adenosine, serving as a critical checkpoint in tumor microenvironments and inflammatory niches – for research & development in cancer immunotherapy (overcoming adenosine-mediated T cell suppression via CD73 blockade), autoimmune/inflammatory disorders (e.g., rheumatoid arthritis, IBD), tissue repair mechanisms (modulating epithelial barrier function) with key advantages including dose-dependent adenosine flux control (vs. static recombinant protein systems) and compatibility with LNP delivery for in vivo mechanistic studies

Manufactured by Levostar´s monoPlasmid technology

Designed for optimal expression results:

• Enhanced polyA(+) Stability – for preventing RNA degradation

• Cap1 Structure – for optimal ribosome recruiting

• Carefully selected UTR Regions – to enhance translation initiation

N1-Methylpseudouridine Modification

For enhanced mRNA stability and decreased anti-mRNA immune response Levostar´s catalog mRNAs are in vitro transcribed using N1-Methylpseudouridine (N1-Me-Ψ, N1mpU) by default. If you prefer unmodified mRNA or you require a different modification, please contact us.

Quality Standards: Tested for high purity, low endotoxin,  low residual dsRNA & plasmid template DNA, high capping efficiency, and proper polyA(+)-length.

Contents:  1 mg mRNA, lyophilized          – also available in solution (1 mg/ml), please inquire

Shipping and Storage:   Lyophilized mRNA shipped at room temperature (≤ 25°C). Store at –80°C upon dissolving.

For other mRNA quantities than 1 mg including bulk offers please contact us for pricing

mRNA may also be ordered already encapsulated in Lipid Nanoparticles for high efficiency mRNA delivery – please contact us for a quotation.

For mRNA encapsulation in your lab and subsequent in vitro mRNA transfection or in vivo mRNA delivery we offer Levostar´s portfolio of NeoLNP Kits.