This kit includes 10 lentiviral constructs with barcodes flanked by common primer sequences to facilitate PCR amplifications. After transduction and antibiotic selection of cells, barcodes can be amplified and sequenced to estimate the number of cells present from a specifically labeled population. In this way, multiple cell populations can be labeled, mixed, growth together, and then the representative numbers of descendant cells from each population can be determined.
Each kit contains 1 x 10^7 TU of 10 individual pre-packaged lentiviral particles to label millions of cells. The barcode constructs in this kit are selectable by puromycin and also have an RFP marker so cells containing barcodes will fluoresce red.