CloneTracker 10-Barcode Cell Labeling Kit Set 2 (RFP/Puro constructs, plasmids)- 10 different sequences

Description

This kit includes 10 lentiviral constructs with barcodes flanked by common primer sequences to facilitate PCR amplifications. After transduction and antibiotic selection of cells, barcodes can be amplified and sequenced to estimate the number of cells present from a specifically labeled population. In this way, multiple cell populations can be labeled, mixed, growth together, and then the representative numbers of descendant cells from each population can be determined.
Each kit contains 10 x 25 ug lentiviral plasmid DNA. The barcode constructs in this kit are different from kit BCRPP10-P, are selectable by puromycin and also have an RFP marker so cells containing barcodes will fluoresce red.