CloneTracker XP 10M Barcode-5′ Library in pScribe9.2-eGFP-Blast (DNA)

Description

Clonetracker XP Lentiviral Barcode Library

• Label large cell populations of any type by lentiviral barcode library transduction
• Assess how cell heterogeneity changes in response to drugs, differentiation, or disease progression (e.g. metastasis)
• Readout by gDNA NGS and Single-Cell RNA-seq, enabled by barcode placement in the 5´UTR of the resistance gene transcript

Track the fate of cell clones over a course of drug treatment or during differentiation, tumorigenesis or other processes. This 10M barcode lentiviral library enables you to easily label more than 2 million cells with heritable unique 5´ UTR RNA-expressed barcodes that are readily detected in RNA-Seq and single-cell expression analysis as well as in the genomic DNA fraction. The barcode location in the 5´ UTR enables SMART-based cDNA synthesis.

Cellecta’s CloneTracker XP™ Barcode-5’ Libraries are pooled barcode libraries that enable the tracking of individual clones derived from a population of cells using either RNA-Seq or PCR/NGS from genomic DNA. This library provided in a lentiviral vector backbone contains about 10 million constructs, each expressing a unique barcode sequence. When a limited cell population is transduced with this barcode library, the barcodes integrate into the genomic DNA of the cells. The result is a founder population where almost every cell has a different DNA-sequenceable barcode that is both integrated into its DNA and expressed on an RNA transcript. Since the barcode is stably integrated, it is passed onto any cell progeny when genomic DNA is replicated. This feature enables identification of all the progeny derived from each individual cell.
When used in conjunction with single-cell RNA-Seq (scRNA-Seq), the expressed barcode may be used to identify expression profiles and activated genes in different cells so that distinct clonal populations of cells from a single progenitor, as well as sub-populations of cells with distinct pathway activations or expression profiles, can be readily identified.
The specially-designed, optimized barcodes facilitate Next-Gen Sequencing (NGS) data analysis and barcode identification. Using the Illumina NGS platform, barcode sequences can be identified either by RNA-Seq or PCR/NGS from genomic DNA and converted to lists with enumerated data.