Co-transcriptional Cap1 mRNA Synthesis Kit

Description

Complete IVT Kit for the co-transcriptional production of Cap1 mRNA

• Convenient one-step protocol for producing Cap1 mRNA
• High transcription yield
• High purity of transcribed product
• High capping efficiency

Co-transcriptional capping is a method for introducing cap structures during in vitro transcription, primarily used to enhance mRNA stability and translation efficiency. The co-transcriptional Cap1 mRNA synthesis kit utilizes T7 RNA polymerase to transcribe DNA sequences downstream of a T7 promoter, using linear double-stranded DNA containing the T7 promoter sequence and an AG initiation sequence as the template, NTPs as substrates (including N1-Me-pUTP if desired), and the cap analog Cap AG(3’Acm) to synthesize mRNA with a 5′-m7G Cap1 structure.

An input of 1 μg of linearized template DNA yields 150-200 μg of Cap1 mRNA

Kit Contents (sufficient for 50 IVT Reactions):

ATP Solution(100 mM)  100 μL
GTP Solution(100 mM)  100 μL
CTP Solution(100 mM)  100 μL
UTP Solution(100 mM)  100 μL
N1-Me-pUTP Solution(100 mM)  100μL
T7 RNA Polymerase  100 μL
RNase Inhibitor(40 U/μL)  100 μL
Inorganic Pyrophosphatase(0.1 U/μL)  100 μL
DNase I (2 U/μL)  100μL
Cap AG(3’Acm)(100mM)  100μL
10×Co-transcription Buffer  100μL
Precipitation Reagent  1 mL
RNase-free ddH2O  1 ml