Custom crRNA Design and Synthesis Service for RNP formation with Cas13

Description

crRNA Design and Synthesis Service for RNP formation with Cas13 nucleases

For your target RNA and your choice of Cas13 enzyme for RNP formation

crRNA for complexation with Cas13 proteins enabling CRISPR-based technologies contain a 5´loop responsible for Cas13a activity resp. a 3´loop for Cas13b activity, and the target RNA specific binding sequence.

Please use the custom guide RNA request form to receive a quote which comprises both the design as well as the synthesis of Cas13 crRNA for either CRISPR genome editing or CRISPR-Dx applications.

SignalChem Dx is using a variety of algorithms to determine crRNA sequences with highest efficiency and specificity to precisely bind your RNA target.

Guide RNA Synthesis can be performed by in vitro transcription (IVT) as well as by chemical synthesis, the latter allowing the incorporation of stabilizing modifications such as 2´-O – Methylation. The synthesis scale starts at 2 nmol, and can otherwise be defined by you in a totally flexible manner – just let us know your needs!