sgRNA comprised of 20 bases CRISPR target sequence fused to crRNA/tracrRNA scaffold will be synthesized by in vitro transcription. 20 ug purified and lyophilized synthetic sgRNA will be delivered and can be complexed with Cas9 to form transfectable/injectable RNP after reconstitution.
Just provide details about your target gene(s) and the desired targeting strategy and Macrogen will design and synthesize sgRNA accordingly for you. In case you have already pre-designed the target sequence(s), just let us know the 20 bp sequence(s) in 5´-3`order without PAM sequence for subsequent sgRNA synthesis.
We offer this service at a scaled price structure depending on the total number of synthesized sgRNAs ordered. Get in touch with us to receive a quote tailored to your needs.