DNA Normalization Magnetic Beads (300ng – 2000ng input: NGS Libraries, PCR Products, 500ng – 5000ng input: gDNA) including recovery beads
285,00 €
Description
Magnetic DNA Normalization Beads – Normalize NGS Libraries, PCR Products, or Genomic DNAs
Bead-based DNA Normalization
- Consistent DNA quantity normalization of various sources in excellent dynamic range
- Easy and efficient protocol – no centrifugation, no filtering
- No more fluorescent quantitation needed
- Cost and Time Saving
- Removal of unwanted components and impurities
- Elution in as low as 20 µl without yield loss
- Ideal for High Throughput Sample Processing
| NGS Library, PCR Fragment, Sheared Genomic DNA | Genomic DNA | |
| DNA Input | 300 ng – 2000 ng | 500 ng – 5000 ng |
| Normalized Range | 100 ng – 180 ng | 150 ng – 270 ng |
| Elution Volume | >20 ul | >20 ul |
| Unbound DNA Recovery | Yes (Cat.# 40072) | Yes (Cat.# 40072) |
During bead-based normalization, DNA samples of varying concentrations are bound to BioDynami´s magnetic DNA normalization beads characterized by a defined limited DNA binding capacity. Bound DNAs are subsequently eluted off the beads at approximately the same concentration for each sample.
This kit version additionally includes recovery beads for the recovery of unbound excess DNA during DNA normalization.
Applications of BioDynami´s Magnetic DNA Normalization Beads:
NGS Library Normalization
Magnetic bead-based NGS library normalization provides significant cost and time savings especially when processing many samples.
This efficient, time-saving alternative bypasses time-consuming and costly library quantitation measurements using fluorescence and/or fragment analyzer instruments. Moreover, manual pipetting steps including dilutions before loading NGS libraries onto the sequencer are no longer necessary.
Normalization of NGS libraries: The input NGS library amount and normalized NGS library amount are shown.
Normalization of Genomic DNA and of PCR Products
Varying amounts of genomic DNA (including sheared genomic DNA fragments in different samples) as well as of PCR Products can easily and conveniently be normalized to uniform DNA concentrations by BioDynami´s Magnetic DNA Normalization Beads.
Normalization of genomic DNA samples: The input genomic DNA amount and normalized genomic DNA amount are shown.
Kit Components: 4.8 ml Magnetic DNA Normalization Beads, 9.6 ml Elution Buffer, 4.8 ml Magnetic Recovery Beads – Sufficient for 96 Runs (based on 50 ul sample volumes)
