DriverMap EXP Human Genome-Wide 19K Expression Profiling Kit (96 multiplex) for dried blood microsamples

Description

DriverMap EXP Targeted RNA-seq Kit for Human Genome Wide Expression Profiling from dried blood microsamples

The DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) for dried blood microsamples measures the expression level of almost 19,000 human protein-coding genes by combining highly multiplexed RT-PCR amplification with the depth and precision of Next-Generation Sequencing (NGS) quantitation. It is an easy-to-run, single-tube, single-day assay that can be run from just one drop of dried blood.

This innovative solution for transcriptomic analysis of small volumes of whole blood (e.g. 30 μl) is a convenient and reliable way of obtaining high-throughput gene expression data for monitoring gene regulation and expression for biomarker and drug discovery applications. With this method, easy sample collection from remote locations without access to traditional blood collection facilities is possible.

The development of the DriverMap™ EXP hGW19K Assay involved extensive optimization and experimental validation of tens of thousands of primer sets to identify a pool of primers that could be combined in a single multiplex RT-PCR reaction to amplify representative transcript sequences (i.e., conserved transcript regions common to different mRNA isoforms from the same gene) from each of ~19,000 protein-coding genes in the human genome (gene list can be downloaded here). The amplified sequences are then quantified using NGS on the Illumina platform.

DriverMap EXP Targeted RNA-seq Kit for dried blood mirosamples:

• Extremely low input of Total RNA
• No mRNA purification needed
• No rRNA or globin mRNA depletion needed
• High dynamic range
• Cost saving by low sequencing depth
• In addition to all the kit features above, the dried blood microsample kit contains additional reagents including magnetic beads for RNA extraction from Mitra blood microsampler tips

The DriverMap™ EXP hGW19K Assay provides optimal quantitative expression data within a 10⁴ – 10⁵-fold dynamic range from 10-50 ng of total RNA and can be used effectively with as little as 1 ng of RNA. Due to the use of gene specific multiplex RT-PCR primers, it is not necessary to purify polyA mRNA, nor to deplete rRNA or globin mRNA. The targeted RNA-seq kit requires 10x lower sequencing depth than RNA-seq and comes with an alignment software for straightforward analysis of the obtained NGS data – no complex genome wide bioinformatics is necessary.

Note: Index Primers need to be purchased separately – we are offering four different Unique Dual Index (UDI) Primer Sets, each in 96-well plate format, for multiplexing up to 384 samples in one NGS run