DriverMap EXP Human Genome-Wide 19K Expression Profiling Kit, v. 3 (24 multiplex)

Description

DriverMap EXP Targeted RNA-seq Kit for Human Genome Wide Expression Profiling

The DriverMap™ EXP Human Genome-wide 19K Profiling Kit (hGW19K) measures the expression level of almost 19,000 human protein-coding genes by combining highly multiplexed RT-PCR amplification with the depth and precision of Next-Generation Sequencing (NGS) quantitation. It is an easy-to-run, single-tube, single-day assay that can be run directly from total RNA isolated from cells, tissue, or blood, or directly from cellular extract using the DirectCell™ Protocol.

The development of the DriverMap™ EXP hGW19K Assay involved extensive optimization and experimental validation of tens of thousands of primer sets to identify a pool of primers that could be combined in a single multiplex RT-PCR reaction to amplify representative transcript sequences (i.e., conserved transcript regions common to different mRNA isoforms from the same gene) from each of ~19,000 protein-coding genes in the human genome (gene list can be downloaded here). The amplified sequences are then quantified using NGS on the Illumina platform.

DriverMap EXP Targeted RNA-seq Kit:

• Extremely low input of Total RNA
• No mRNA purification needed
• No rRNA or globin mRNA depletion needed
• High dynamic range
• Cost saving by low sequencing depth
• Easy readout

The DriverMap™ EXP hGW19K Assay provides optimal quantitative expression data within a 10⁴ – 10⁵-fold dynamic range from 10-50 ng of total RNA and can be used effectively with as little as 1 ng of RNA. Due to the use of gene specific multiplex RT-PCR primers, it is not necessary to purify polyA mRNA, nor to deplete rRNA or globin mRNA. The targeted RNA-seq kit requires 10x lower sequencing depth than RNA-seq and comes with an alignment software for straightforward analysis of the obtained NGS data – no complex genome wide bioinformatics is necessary.