DriverMap TCR Single-Cell AIRR-seq Kit / Mouse TCR Chain Pairing Assay

Description

Identify paired full-length TCR sequences in single mouse T cells sorted in a 96-well plate

Easy-to-run, single-day scAIRR-seq assay that uses multiplex RT-PCR and NGS technology to profile paired TCR sequences at single-cell resolution

The DriverMap™ Single-cell Adaptive Immune Receptor (scAIR) Sequencing Assay enables identification of TCR paired full-length (V(D)J) immune receptor sequences of single T cells sorted in a 96-well plate. The paired full-length receptor sequence information is necessary to clone and express functional TCRs for follow-up antigen discovery studies and the development of cell-based therapies.

• The assay works with FACS-sorted cells in a 96-well plate without needing special microfluidic equipment or complex split-pool barcoding workflows

• Simultaneously amplify—in a single multiplex RT-PCR reaction—full-length (CDR1-CDR2-CDR3) regions for alpha and beta (TRA and TRB) TCR chains of single T cells sorted in 96-well plate(s)

• The use of well/cell-specific barcodes and of primers which are highly specific to T-Cell Receptors and incoporate Validation Barcodes (VBC) enables a highly efficient protocol for pooled Multiplex RT-PCR based NGS library preparation

• NGS data can be analyzed with MiXCR, Platforma, and RSEM software packages available online

Kit Description and Contents:

• Profiles paired full-length TCR alpha and beta chain (TRA, TRB) sequences at single-cell resolution
• Includes all the enzymes, reagents, and primers to prepare 8 x 96 samples ready for NGS
• Assay(s) can be run in a single reaction without having to run multiple expensive assays and without any specialized equipment or complex split-pool approaches
• NGS data can be analyzed with MiXCR, Platforma, and RSEM software packages available online