High Molecular Weight Genomic DNA Extraction Kit for Plants (Magnetic Beads) with disposable pestles and tubes

Description

Centrifuge and Column free HMW Genomic DNA Purification from Plants

Extract High Molecular Weight DNA (50 kb – 200 kb) from plants with magnetic beads

• Highly pure, RNA-free HMW gDNA (A260/A280 1.8, A260/A230 1.5-2.5)
• High yields (Up to 10 ug from up to 50 mg tissue)
• Simple magnetic beads method – no centrifuge, no column, no vacuum
• For long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and more
• Kit comes with 50 disposable plastics pestles and tubes designed for plant homogenization

The High Molecular Weight Genomic DNA Extraction Kit (Magnetic Beads) for Plants provides a reliable and fast process for extracting HMW genomic DNA from plants, using Solid Phase Reversible Immobilization (SPRI) magnetic beads. The kit eliminates the tedious centrifuge steps for columns and provides a reliable, fast, and simple approach for high-quality genomic DNA isolation with high binding capacity.

Plant tissues, such as fresh leaves, seeds, pedals, roots, are homogenized using the provided plastics pestles and lysed, then magnetic beads are added to bind plant DNA before the supernatant is removed by magnetic separation. The DNA-binding magnetic beads are washed to remove residual polysaccharides and polyphenols, then resuspended in a buffer followed by magnetic separation to remove supernatant from the DNA-binding beads. The purified plant DNA is eluted in 100 ul Elution Buffer after the washing step. The isolated HMW genomic DNA is free of contaminations such as RNA, polysaccharides, polyphenols, lipids, proteins, and salt.