High Molecular Weight Genomic DNA Extraction Kit for Tissue (Magnetic Beads)

Description

Centrifuge and Column free HMW Genomic DNA Purification from Tissue

Extract High Molecular Weight DNA (50 kb – 150 kb) from tissue with magnetic beads

• Highly pure, RNA-free HMW gDNA (A260/A280 1.8-2.0, A260/A230 2.2-2.5)
• High yields (Up to 30 ug from up to 10 mg tissue)
• Simple magnetic beads method – no centrifuge, no column, no vacuum
• For long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and more

The High Molecular Weight Genomic DNA Extraction Kit (Magnetic Beads) for Tissue provides a reliable and fast process for extracting HMW genomic DNA from tissue, using Solid Phase Reversible Immobilization (SPRI) magnetic beads. The kit eliminates the tedious centrifuge steps for columns and provides a reliable, fast, and simple approach for high-quality genomic DNA isolation with high binding capacity.

Tissues are homogenized and lysed with a lysis buffer, and then mixed with magnetic beads by tube tapping to bind genomic DNA. After mixing and washing steps, high molecular weight genomic DNA is eluted in 100 ul elution buffer. The isolated HMW genomic DNA is free of contaminations such as RNA, proteins, salts, and other impurities.