• Single library with ~165,000 constructs
• Each gene is covered by 8 shRNAs targeting functional domains and conserved transcript regions
• 19,000 human protein coding genes targeted
All three modules of Cellectas Human Genome-Wide (hGW) shRNA Library combined together in a single library pool of ~165,000 constructs.
Cellecta´s 3-module pooled lentiviral Human Genome-wide shRNA Library cloned in pRSI16cb targets nearly all protein-encoding genes of the human genome, and each gene is targeted by 8 hairpins. The library includes shRNA-specific barcodes that provide an alternative to direct sequencing of the shRNA target sequence which is difficult to sequence due to the stem-loop structure.