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Human CRISPRa Dual-sgRNA Library (UbiC) (DNA)-academia

5.425,00 

Quantity: 200 ug

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Description

Genome-wide pooled library of ~105,000 dual-sgRNA constructs each targeting the promoter region of a single human gene (each construct expresses two sgRNA targeting the same gene). Designed to work with deactivated spCas9 (dCas9) complexed with an activator (e.g., dCas9-VPH) to activate gene expression. Each gene is targeted by 5 dual sgRNAs designed against the promoter region (designs based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554). Targeting each gene with two sgRNAs for its promoter region means stronger activation.

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