• Genome-Wide Coverage with a Single 105K Module
• Single library with ~105,000 constructs
• The promoter region of each gene is targeted by 5 sgRNAs in dual combinations of each of the 5 sgRNA designs enhancing gene activation
• All human protein-coding genes covered—approximately 19,000 gene targets
Genome-wide pooled library of ~105,000 sgRNA constructs cloned in the pRSL10-mU6-sg1-hU6-sg2-UbiC-TagRFP-2A-Puro vector targeting the promoter region of ~19,000 protein-coding human genes. Designed to work with deactivated spCas9 (dCas9) complexed with an activator (e.g., dCas9-VPH) to induce gene expression.
The promoter region of each gene is targeted by 5 sgRNAs in dual combinations of each of the 5 sgRNA (design based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554). Targeting genes with dual-sgRNA constructs leads to increased gene activation.