Human CRISPRi Dual-sgRNA Library (UbiC) (DNA)-academia


Quantity: 200 ug

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Genome-wide pooled library of ~105,000 dual-sgRNA constructs each targeting the promoter region of a single human gene (each construct expresses two sgRNA targeting the same gene). Designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Each gene is targeted by 5 dual sgRNAs designed against the promoter region (designs based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554). Targeting each gene with two sgRNAs for its promoter region means stronger repression.

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