Lentiviral particles expressing human CTLA-4 (NCBI ID: NM_005214.4, alternative names: CTLA4, ALPS5, CD152, CELIAC3, GRD4, GSE, IDDM12). The full sequence-verified ORF is natively expressed under an optionally inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present. A Blasticidin–RFP fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance. Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. 200 ul concentrated particles at 1 x 10e8 TFU/ml in PBS are provided. CTLA-4 shRNA and CRISPR knockout constructs are also available – please contact us for more information.
In case you do not find the immunotherapy target construct you are looking for, please contact us – we have many additional premade constructs and can also offer custom synthesis for you.