iSWAB ID Human Forensic DNA Collection Kit (up to 7 ug DNA)

Description

Kit for forensic human DNA collection and preservation from buccal cells, blood spots, sweat, semen, and any other body fluid, & transport/storage at ambient temperatures – all in the same tube

Kit contents:1 sterile cotton tipped applicator bag (containing 1 swab) , 1 barcode-labeled iSWAB-ID tube (400 ul iSWAB preservation solution), 1 extra barcode label, 1 plastic “biohazard” bag with absorbent pad, 1 “Exempt Human Specimen” label, and a box which may be used to mail back the sample

Please let us know the required kit quantity to receive a price quote.

• Collect – Recover – Concentrate – Stablize – Record – Transport – Store – Analyze: All in a Single tube
• Can be used for the collection of both, non-invasive reference samples (DNA and protein from buccal cells) and evidentiary touch-DNA samples (blood spots, sweat, semen, and any other body fluid stains)
• Concentrate and Stabilize: Complete release and stabilization of the sample from the swab into the iSWAB-ID tube at the point of collection (no drying time required)
• Record: Pre-barcoded components to maintain chain of custody
• Transport and Store: Transport and long term storage over years, at at room temperature
• Analyze: High DNA recovery allows for archiving as well as multiple runs of Direct PCR, Genotyping and DNA sequencing (Sanger & NGS)

The collection of biometric and crime scene samples is a key step in any forensic investigation process. As critical as this step is, however, traditional collection tools such as swabs and FTA cards leave significant room for error or unusable samples. After collection, inadequate resources can often lead to a backlog of several weeks or months to process the samples therefore increasing the possibility of yielding invalid results. Factors which can negatively impact sample integrity include improper storage and transport leading to bacterial or mold growth, DNases released by collected cells can degrade DNA rapidly, and overdrying of the sample can result in the irreversible binding of DNA. In addition, there is often not enough evidence to support multiple assays.
The iSWAB-ID device represents a significant advancement in sample collection and stabilization technology: DNA is immediately stabilized and preserved at the point of collection and iSWAB-ID allows shipment as well as long-term storage at room temperature maintaining integrity of the samples over years. This system allows for maximizing sample recovery and obtaining human DNA compatible with the whole range of ID profiling assays.

Applications

Devices for collecting forensic DNA samples including reference samples, buccal cells, blood spots, sweat, semen, and any other bodily fluids.
Forensics, ID Profiling Assays.

Product Description

iSWAB-ID is an efficient sample collection system for evidentiary and reference DNA samples, which enables long-term room temperature stabilization of collected samples at the point of the collection while ensuring a proper chain of custody. Typically yields 2-7μg (iSWAB-ID) of DNA with <3% bacterial contamination. This system allows for maximizing sample recovery and obtaining human DNA compatible with ID profiling assays. DNA extraction can be performed using any commercially available whole-blood extraction chemistry. Intended Use

iSWAB-ID Human DNA Collection Kit is a DNA sample collection device for the collection, stabilization, concentration, storage, and transport of evidentiary DNA samples. It is a non-invasive swab-based sampling applicator and is intended to be used for a sample collection from various sample types including Touch DNA, Reference Samples, buccal cells, blood spots, sweat, semen, and any other bodily fluids. iSWAB-ID has been shown to efficiently recover and stabilize DNA of forensic significance at the point of collection.

Indication for Use

iSWAB™-ID is an efficient sample collection system for evidentiary and reference DNA samples, which enables long-term room temperature stabilization of the collected sample at the point of the collection while ensuring a proper chain of custody. This system allows for maximizing sample recovery and obtaining human DNA compatible with ID profiling assays. DNA extraction can be performed using any commercially available whole-blood extraction chemistry. Collecting samples with iSWAB™-ID allows for multiple runs for analysis and archiving purposes. Collected samples are stable for 3 years at room temperature storage (based on accelerated stability testing).

Storage and Stability

Pre-collection: 3 years from date of manufacture between 15-30° C.

Transport temperature, post-collection: 15-30° C

Sample stability and storage, post-collection: 60 days real time and 3 years accelerated time. If longer storage time is required, collected samples can be stored at -20 to -80° C.

Downstream Processing:
Any commercially available kit for extracting human gDNA from whole blood (either manually or automation-enabled) can be used without any further modifications
Direct PCR-STR Kits tested to be compatible with iSWAB-ID: AmpFLSTR® Identifiler® Direct PCR Amplification Kit (Thermo), PowerPlex® Fusion and Y23 Systems (Promega)

See also MAWI DNA Technologies  Extraction Methods Overview for further information.