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iSWAB RNA Collection Kit

780,00 

Quantity: 20 kits

    Please contact us for a quotation

     


    Description

    Kit for non-invasive RNA collection and room temperature RNA preservation, transport & storage – all in the same tube

    Kit contents: 4 sterile cotton tipped applicator bags (each containing 1 swab), 1 barcode-labeled iSWAB-RNA tube (1ml iSWAB-RNA preservation solution), 1 extra barcode label, 1 plastic “biohazard” bag with absorbent pad, 1 “Exempt Human Specimen” label, and a box which may be used to mail back the sample

    Please let us know the required kit quantity to receive a price quote.

    • Collect – Stabilize – Concentrate – Transport – Store – Extract: All in a Single Tube
    • Extended RNA stability (up to 4 weeks at room temp, several years at -20 C and -80 C)
    • High quality RNA for downstream applications such as RT-PCR, Microarray and RNA-seq for gene expression analysis
    • Simple, fast and convenient sample collection: Self collection or assisted collection with high simplicity and ease in less than 5 minutes
    • Swab-free sample transport: Improved sample integrity and decreased sample processing time
    • Accommodate for samples from all age groups: Suitable for all population segments including infants, toddlers, and elderly
    • Traceable: LIMS compatible unique barcodes for each iSWAB tube ensures efficient traceability and storage
    • Room temperature stablilty for 4 weeks: Reduce sample storage and transport costs by eliminating cold chain
    • Scalable and easy to process: Manual and automation friendly sample processing

    RNA Collection, Concentration, and High Quality RNA Stabilization at Room Temperature for applications such as qRT-PCR, RT-PCR, RNA-Seq, Microarrays, Northern Blot, Nuclease Protection Assays, cDNA library construction etc.:

    The iSWAB-RNA device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance. The need to collect blood results in a very limited shelf life, forcing labs to invest in cold storage infrastructure or commit resources to immediate sample processing. Other options for non-invasive collection also suffer from short shelf life storage as well as low quality and unusable RNA.

    Applications

    Devices for collecting human RNA samples via buccal cells or mammalian cell RNA samples with cytobrush
    Transcriptomics, Gene Expression: qPCR and RNA-based sequencing (RNAseq) , microarray, Northern Blot, Nuclease protection assays, cDNA library construction, Mass spectrometry, ELISA, Western blotting, tissue biopsy stabilization.

    Product Description

    The iSWAB-RNAv2 device is a non-invasive technology for the collection, concentration, and stabilization of intact buccal cells or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. The design of the iSWAB collection device allows for the maximum release of collected samples from multiple swabs or brushes into a single device, to achieve a more concentrated sample. Self-collection or assisted collection can be achieved in less than 2 minutes and is suitable for all population segments including infants, toddlers, and elderlies.

    Intended Use

    iSWAB RNA v2 Collection Kit is a RNA sample collection device for the collection, stabilization, concentration, transport and storage of RNA samples. It is a non-invasive swab-based sampling applicator and is intended to be used for sample collection from various sample types including intact buccal cells and/or any mammalian cells collected with a swab or cytobrush.

    Indication for Use

    The iSWAB-RNA-v2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush. Allows for real time ambient stabilization of total RNA from the point of collection to processing. The iSWAB™-RNA-v2 maintains RNA stability and protects RNA from degradation. The device allows extended shelf life stability (up to 4 weeks at room temp, several years at -20 C and -80 C), reduced processing time, and minimal storage footprint.

    Storage and Stability

    Pre-collection: 18 months from date of manufacture between 15-30° C.
    Transport temperature, post-collection: 15-30° C.
    Sample stability and storage, post-collection: viable cells and total RNA are stabilized for up to 4 weeks at at 15-30° C; if longer storage time is required, collected samples can be stored at -20 to -80° C for several years.

    Downstream Processing:
    Use 600 ul of thoroughly mixed iSWAB-RNA suspension with MagBio´s bead-based automation compatible HighPrep Total RNA Plus Kit (Cat. Nos. HPTOR-R5, HPTOR-R50, HPTOR-R100, HPTOR-R400) incl. DNAse treatment
    Use 300 ul of thoroughly mixed iSWAB-RNA suspension with Zymo´s Quick-RNA™ MiniPrep Plus Kit (Cat. Nos. R1057 and R1058) without DNAse treatment

    See also MAWI DNA Technologies  Extraction Methods Overview for further information.

    iSWAB Direct RT-PCR:
    The proprietary iSWAB-RNA buffer is a unique formulation that not only stabilizes the samples at the point of collection, but performs a gentle lysis which slowly releases RNA from the cells into the buffer over a period of several hours.
    This feature enables the mixture to be used in a direct-to RT-PCR application, in which the extraction step is skipped entirely.
    For more complex downstream applications such as Next Generation Sequencing or microarrays, we still recommend performing a standard RNA extraction as described above.

    The procedure for preparing iSWAB samples to be used directly in RT-PCR is as follows:
    1. Collect buccal sample with iSWAB-RNA
    2. Incubate the collected iSWAB-RNA at least 3 hrs at room temperature before processing in direct RT-PCR
    3. Centrifuge the collected iSWAB-RNA for 2 minutes at 14000 rpm
    4. Take 1uL of the clarified iSWAB supernatant and dilute in nuclease-free water to 1:16
    5. Take 1 or 2 uL (depending on your required sample input for your RT-PCR protocol) from the iSWAB diluted sample and apply to your direct or standard RT-PCR protocol

    Shipping Conditions: Room Temperature
    Storage Temperature: Room Temperature