The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam). Expression is constitutive and driven by the CMV promoter. A Kozak sequence should be added to the 5’ end of the gene insert for efficient translation. Target proteins are tagged with C-terminal eGFP, CL7, and His8 tags. PreScission Protease cleavage removes all tags.