MGC premier Human ORFeome Collaboration Collection, subset: ORFs with stop codon

Description

ORFeome Collaboration Human ORF Clone Collection for Gene Overexpression

Arrayed Collection of Human ORF Clones for functional studies of the Human Proteome

Subset: ORFs with stop codon facilitating the synthesis of native proteins

• Full-length, well annotated and sequence verified ORFs

• Constructed in Gateway® Entry vectors enabling transfer into any type of Gateway® compatible destination vector

The MGC premier human ORFeome Collaboration Library currently represents 9,804 genes with 16,581 clones. The majority of the ORFeome collaboration targets were generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (DFCI-CCSB). These full-length, annotated and sequence verified ORFs contain the coding sequences located between the initiation and termination codons, excluding the 5′ and 3′ mRNA untranslated regions, and derive from the MGC premier collection of full-length human cDNA clones. The ORFS have been transferred into Gateway Entry vectors as a ready to use resource for recombinant protein expression. For additional convenience and versatility, the ORF clones are available in two formats, with and without stop codons. The ORF clones without stop codons facilitate the synthesis of either C- or N- terminal fusion proteins and clones with stop codons enable the synthesis of native proteins in addition to N-terminal fusion proteins.

CONTENTS:

The ORFeome Collaboration Library Subset of ORF Clones with stop codons, is delivered as 96-well glycerol stock plates (with lids) – 73 plates total. Plates are sealed with aluminum sealing tape and are shipped on dry ice.

The plates should be stored at -80C upon arrival.